The Substrate Analog, Bromopyruvate, as a Bridging Agent for the Active Site of 2-Keto-3-deoxy-6-phosphogluconic Aldolase
نویسندگان
چکیده
منابع مشابه
The Mechanism of 2-keto-3-deoxy-6-phosphogluconic Aldolase.
In the original study of 2-keto-3-deoxy-6-phosphogluconic aldolase (I), and in subsequent experiments, the net synthesis of 2-keto-3-deoxy-6-phosphogluconate from the cleavage products could not be demonstrated. The present experiments were undertaken to clarify this point and to test the conformity of the reaction catalyzed by 2-keto-3-deoxy-6-phosphogluconic aldolase to the general characteri...
متن کاملThe Substrate Analog, Bromopyruvate, as a Bridging Agent for the Active Site of Z-Keto-3-deoxy-6-phosphogluconic Aldolase CHEMICAL EVIDEKCE FOR A CARBOXYLATE ADJACEPU’T TO THE SCHIFF’S BASE-FORMING LYSINE*
The enzyme, 2-keto-3-deoxy-6-phosphogluconic aldolase is inactivated by the substrate analog, bromopyruvate. Inactivation results from either an active site carboxyl group or a cysteine being alkylated by bromopyruvate. Treatment of enzyme inactivated by carboxylate ester formation with borohydride produces the secondary amine resulting from reduction of a Schiff’s base between the covalently f...
متن کاملGDP-4-keto-6-deoxy-D-mannose 3-dehydratase, accommodating a sugar substrate in the active site.
Colitose is a dideoxysugar found in the O-antigen of the lipopolysaccharide that coats the outer membrane of some Gram-negative bacteria. Four enzymes are required for its production starting from D-mannose-1-phosphate and GTP. The focus of this investigation is GDP-4-keto-6-deoxy-D-mannose 3-dehydratase or ColD, which catalyzes the removal of the C3'-hydroxyl group from GDP-4-keto-6-deoxymanno...
متن کاملIdentification of 2-keto-3-deoxy-d-Gluconate Kinase and 2-keto-3-deoxy-d-Phosphogluconate Aldolase in an Alginate-Assimilating Bacterium, Flavobacterium sp. Strain UMI-01
Recently, we identified an alginate-assimilating gene cluster in the genome of Flavobacterium sp. strain UMI-01, a member of Bacteroidetes. Alginate lyase genes and a 4-deoxy-l-erythro-5-hexoseulose uronic acid (DEH) reductase gene in the cluster have already been characterized; however, 2-keto-3-deoxy-d-gluconate (KDG) kinase and 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase genes, i.e., f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43344-9